The influence of P‐light chain phosphorylation by myosin light chain kinase on the calcium sensitivity of chemically skinned heart fibres

FEBS Letters

Morano, I.¹  Rüegg, J.C.¹  Hofmann, F.²  Zimmer, M.² 

[1] II. Physiologisches Institut der Universität Heidelberg, Im Neuenheimer Feld 326, 6900 Heidelberg, FRG;Physiologische Chemie der Medizinischen Fakultät Hamburg, 6650 Hamburg/Soar, FRG

关键词: Myosin light chain kinase;    P-light chain;    Phosphorylation;    Chemically skinned heart fibre;   

DOI  :  10.1016/0014-5793(85)81027-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphorylation of the P-light chain of myosin might be involved in the regulation of cardiac contractility. Thus an enhanced phosphorylation level of the P-light chain catalyzed by Ca²⁺-calmodulin-dependent myosin light chain kinase (MLCK) increased significantly the Ca²⁺ sensitivity of chemically skinned ventricular fibre bundles of the pig. This effect was reversible. Whereas force development at submaximal Ca²⁺ concentration (pCa 5.5) increased by ~ 50% in the presence of MLCK, maximum tension achieved at maximum Ca²⁺-concentration (pCa 4.3) was not affected.

【 授权许可】

Unknown