A wasp venom mastoparan‐induced polyphosphoinositide breakdown in rat peritoneal mast cells

FEBS Letters

Tohmatsu, Toyohiko¹  Nakashima, Shigeru¹  Kuroda, Yoichiro³  Okano, Yukio¹  Saito, Kazuki²  Takagi, Hajime¹  Nozawa, Yoshinori¹ 

[1] Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu 500, Japan;Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;Department of Neurochemistry, Tokyo Metropolitan Institute for Neurosciences, 2-6 Musashidai, Fuchu, Tokyo 183, Japan

关键词: Mast cell;    Phosphoinositide;    Calcium;    Mastoparan;    PI;    phosphatidylinositol;    PIP;    phosphatidylinositol 4-phosphate;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    IP;    inositol phosphate;    IP2;    inositol bisphosphate;    Ip3;    inositol trisphosphate;    DG;    diacylglycerol;    PA;    phosphatidic acid;    PC;    phosphatidylcholine;    PE;    phosphatidylethanolamine;    GTP;    guanosine 5 '-triphosphate;    MCM;    mast cell medium;    BSA;    bovine serum albumin;   

DOI  :  10.1016/0014-5793(85)80403-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The phospholipid metabolism of rat peritoneal mast cells stimulated with mastoparan, a secretagogue purified from wasp venom, was investigated. Mastoparan at 20 caused a rapid secretion of histamine. Mastoparan induced a transient decrease of phosphatidylinositol 4,5-bisphosphate on ³²P labeling and generation of a water-soluble degradation product, inositol trisphosphate on [³H]inositol labeling, suggesting the activation of phospholipase C upon stimulation.

【 授权许可】

Unknown