Heparin induces Ca2+ release from the terminal cysterns of skeletal muscle sarcoplasmic reticulum

FEBS Letters

Kozlov, Yuri P.¹  Men'shikova, Elizaveta V.¹  Ritov, Vladimir B.¹ 

[1] School of Biology, M.V. Lomonosov Moscow State University, Moscow 119899, USSR

关键词: Sarcoplasmic reticulum;    Ca2+ transport;    Chlorotetracycline fluorescence technique;    Heparin;   

DOI  :  10.1016/0014-5793(85)80878-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a Ca²⁺-selective electrode and the chlorotetracycline fluorescence technique, the effects of heparin on Ca²⁺ transport in the sarcoplasmic reticulum (SR) of skeletal muscles in the absence of oxalate were investigated. It was shown that heparin (0.5–10 ) causes a rapid release of 40–50 nmol protein from the terminal cystern SR vesicles bound to 130–150 protein of Ca²⁺ in the presence of ATP. However, heparin has practically no effect on the longitudinal cystern fraction of SR. The effects of heparin can be prevented by ruthenium red. No influence of heparin is observed in the case of the Ca²⁺-induced release of Ca²⁺ from the terminal cysterns. When the Ca²⁺ release is induced by heparin, no Ca²⁺-induced release of Ca²⁺ takes place.

【 授权许可】

Unknown