Excitation‐contraction coupling in skeletal muscle fibers injected with the InsP3blocker, heparin

FEBS Letters

Baylor, S.M.¹  Pape, P.C.¹  Konishi, M.¹  Somlyo, A.P.¹ 

[1] Department of Physiology and Pennsylvania Muscle Institute, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6085, USA

关键词: Excitation-contraction coupling;    Inositol trisphosphate;    Heparin;    Sarcoplasmic reticulum;    Ca2+;    (Skeletal muscle);   

DOI  :  10.1016/0014-5793(88)81233-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Heparin, an inhibitor of inositol trisphosphate (InsP₃)-induced Ca²⁺ release in smooth muscle and non-muscle cells, was injected into intact frog skeletal muscle fibres. Ca²⁺ release from the sarcoplasmic reticulum was elicited by the normal action potential mechanism and monitored by both fura-2 fluorescence and an intrinsic birefringence signal. Both optical signals, and hence Ca²⁺ release, were unaffected by high concentrations of heparin. This result argues against a major physiological role of InsP₃ as a chemical messenger of excitation-contraction coupling in skeletal muscle.

【 授权许可】

Unknown