Isolation of the epidermal growth factor from the shrew submaxillary gland

FEBS Letters

Tam, Y.Y.¹  Kong, Y.C.¹  Yip, T.T.¹  Porath, J.²  Belew, M.C.² 

[1] Department of Biochemistry, University of Hong Kong, Shatin, NT, Hong Kong China;Institute of Biochemistry, Biomedical Center, University of Uppsala, Uppsala, Sweden

关键词: Shrew;    EGF;    Immobilized metal ion affinity chromatography;    Radioreceptor assay;    Ni-TED;    Ni2+ immobilized on tricarboxymethylethylenediamine (TED) covalently linked to Sepharose 6B;    IMAC;    immobilized metal ion affinity chromatography;   

DOI  :  10.1016/0014-5793(85)81273-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The epidermal growth factor (EGF) from the submaxillary glands of the Chinese Shrew (Suncus murinus) is purified to apparent homogeneity by using a sequence of four chromatographic steps, viz. gel filtration on Sephacryl S-200, affinity chromatography on immobilized Ni, hydrophobic interaction on phenyl-Sepharose CL-4B and reverse-phase HPLC. An 800-fold increase in specific activity and an overal recovery of 46% were achieved. The most effective step in its purification is the successful use of immobilized metal ion affinity chromatography (IMAC). This method was very selective, reproducible and requires a minimum of sample pre-treatment prior to chromatography.

【 授权许可】

Unknown