Activity of human, bovine and porcine platelet‐derived growth factor in a radioreceptor assay with human placental membrane protein

FEBS Letters

Czyrski, Józef A.²  Gawlikowski, Wojciech¹ 

[1] Laboratory of Radioimmunology, Military Hospital, 53-115 Wrocław, ul. Czerska 5, Poland;Laboratory of Tumor Virology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wrocław, ul. Czerska 12 Poland

关键词: Platelet-derived growth factor;    Membrane protein;    Radioreceptor assay;    (Human placenta);   

DOI  :  10.1016/0014-5793(87)80246-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Radioiodinated human platelet-derived growth factor (¹²⁵I-PDGF), and unlabeled human (Hu), bovine (Bo), and porcine (Po) PDGF were used to study PDGF receptors in human term placental membrane preparations. The binding of ¹²⁵I-Hu-PDGF was inhibited by unlabeled preparations. Half-maximal occupancy of the binding sites occurred at 7–9 nM of Hu-, Bo- or Po-PDGF. Scatchard analysis of the binding data indicated a single class of receptors having a K _d value of about 1.1–1.2 × 10⁻¹⁰ M. Thus, the sensitive radioreceptor assay does not detect any species specificity of PDGF.

【 授权许可】

Unknown