Subclass‐specific antibody‐dependent binding of macrophages to supported planar lipid monolayer membranes

FEBS Letters

Nakanishi, Mamoru¹  Kimura, Kazuko¹ 

[1] Faculty of Pharmaceutical Sciences, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan

关键词: Lipid monolayer Planar membrane Lipid hapten Macrophage IgG subclass Fc receptor;    DMPC;    dimyristoylphosphatidylcholine;    DPPC;    dipalmitoylphosphatidylcholine;    PBS;    phosphate-buffered saline;    TNP-Cap-DPPE;    trinitrophenylaminocaproylphosphatidylethanolamine;    PCS;    fetal calf serum;    ELISA;    enzyme-linked immunosorbent assay;   

DOI  :  10.1016/0014-5793(85)81216-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Subclass-specific antibody-dependent binding of macrophages to supported planar lipid monolayers has been studied. The binding of the P388D₁, macrophage-like cell line to planar DMPC or DPPC monolayers was dependent on IgC subclasses and hapten concentrations. The binding efficiencies were as follows on both ‘solid’ and ‘fluid’ lipid monolayer membranes; IgGI = IgG2a>IgG2b>IgG3 = IgA. These results suggest that the present system is very useful for studying the mechanisms of the transmembrane signal triggered by Fc receptors of macrophages.

【 授权许可】

Unknown