Lipid interaction of Tetanus neurotoxin A calorimetric and fluorescence spectroscopy study

FEBS Letters

Montecucco, Cesare²  Calappi, Emanuela¹  Masserini, Massimo¹  Schiavo, Giampietro²  Tettamanti, Guido¹ 

[1] Department of Medical Chemistry and Biochemistry, University of Milano, Milano, Italy;Institute of General Pathology, University of Padua, Padua, Italy

关键词: Tetanus toxin;    Ganglioside microdomain;    TeNT;    Tetanus toxin;    DPPC;    Dipalmitoylphosphatidylcholine;    DMPC;    dimyristoylphosphatidylcholine;    DPH;    1.6-diphenyl-1;    3;    5-hexatriene;    Pyrene-PC;    pyrenedecanoylpalmitoylphosphatidylcholine;    DPPA;    dipalmitoylphosphatidic acid;    DMPA;    dimyristoyl-phosphatidic acid;    Tm;    Gel to liquid crystalline temperature transition;    DSC;    Differential Scanning Calorimetry;   

DOI  :  10.1016/0014-5793(92)81074-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interaction of Tetunus toxin with phospholipid vesicles containing gangliosides (GD1a, GD1b or GT1b) or phosphatidic acid has been investigated at neutral or acidic pH. Change in the thermotropic properties of the vesicles occurred only after addition of the toxin at acidic pH, and led to surface binding or membrane insertion of the protein, dependent on the physical state of the membrane. Most remarkably, toxin addition at acidic pH to dipalmitoyl-phosphatidylcholine vesicles containing GT1b ganglioside, caused formation of ganglioside microdomains on the vesicle surface.

【 授权许可】

Unknown