Initiation of flagellar rotation in Rhodopseudomonas sphaeroides

FEBS Letters

Evans, Michael C.W.¹  Armitage, Judith P.¹ 

[1] Department of Botany and Microbiology, University College London, London, WC1E 6BT, England

关键词: Chemiosmosis;    Motility;    Uncoupling agent;    Photosynthesis;    Respiration;    Mes;    4-morpholineethanesulphonic acid;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    DNP;    dinitrophenol;    S13;    5-chloro-3-t-butyl-2'-nitrosalicylanilide;    pmf;    proton-motive force;   

DOI  :  10.1016/0014-5793(85)81346-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The collapse of the proton-motive force (pmf) in dark-incubated Rps. sphaeroides by the addition of low concentrations of anionic uncouplers caused a loss of motility. When added to bacteria under continuous illumination, the same uncoupler concentration had no effect on motility. Re-illumination of the nonmotile, dark-incubated bacteria caused a very fast increase in the pmf, but there was a delay before pmf-driven flagellar rotation was initiated. The length of the delay, which could be many minutes, was shown to be dependent on the type of uncoupler used, the ΔpH component of the pmf and to be independent of the overall size of the pmf or the temperature. It is concluded that the delay in reinitiation of motility was due to the direct interaction of anionic uncouplers with sites on the flagellar motor proteins.

【 授权许可】

Unknown