Titrations of ATP synthesis with uncoupling agents do not provide evidence of localised high energy intermediates in electron transport phosphorylation in bacterial chromatophores

FEBS Letters

Cotton, N.P.J.¹  Jackson, J.B.¹ 

[1] Department of Biochemistry, University of Birmingham, PO Box 363, Birmingham B15 2TT, England

关键词: ATP synthesis;    Chemiosmosis;    Chromatophore;    Photosynthesis;    Uncoupling agent;    FCCP;    carbonylcyanide-p-trifluoromethoxyphenylydrazone;    SF6847;    3;    5-di-tert-butyl-4-hydroxybenzylidenemalononitrile;   

DOI  :  10.1016/0014-5793(83)80737-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The dependence of light-driven ATP synthesis by chromatophores upon uncoupling agent concentration in the presence and absence of F_o inhibitors was investigated. When experiments were carried out using the procedure described by Hitchens and Kell [Biochem. J. (1983) 212, 25–30] it appeared as though uncouplers were more potent in the presence of F_o inhibitors. This however was the result of uncorrected, time-dependent changes in the rate of ATP synthesis. When the experimental procedure was modified to minimise the effect of time-dependent changes in rate, it was found that uncoupler potency was not significantly changed by the presence of F_o-inhibitors. These results do not discriminate between localised and delocalised models of energy coupling and are entirely consistent with the chemiosmotic hypothesis.

【 授权许可】

Unknown