Monoclonal antibodies to cytochrome P‐450 immunopurify a 45‐kDa protein from a human lymphoblastoid cell line

FEBS Letters

Marietta, Michael A.¹  Friedman, Fred K.¹  Park, Sang Shin¹  Gelboin, Harry V.¹  Robinson, Richard C.¹  Pastewka, Jullia V.¹ 

[1] Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, MD 20205 USA

关键词: Cytochrome P-450;    Aryl hydrocarbon hydroxylase;    Monoclonal antibody;    Immunopurification;    Epitope;    Lymphoblastoid cell;   

DOI  :  10.1016/0014-5793(85)80742-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Monoclonal antibodies (MAbs) to rat liver cytochromes P-450 have previously been used for successful immunopurification of cytochromes P-450 from animal tissues. We now report application of this MAb-based immunopurification technique to the human lymphoblastoid AHH-1 cell line. Immunopurification carried out with 3 different MAbs each yielded a 45-kDa polypeptide. The purified protein contains an MAb-specific epitope present on cytochromes P-450, and may therefore be a human cytochrome P-450.

【 授权许可】

Unknown