cDNA‐directed expression of human thyroid peroxidase

FEBS Letters

Kimura, Shioko¹  Kotani, Tomio²  Ohtaki, Sachiya²  Aoyama, Toshifumi¹ 

[1] Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, MD 20892, USA;Central Laboratory of Clinical Investigation, Miyazaki Medical College Hospital, Kiyotake, Miyazaki 889-16, Japan

关键词: Peroxidase;    cDNA expression;    Vaccinia virus;    Immunopurification;    (Human thyroid);    TPO;    thyroid peroxidase;    TK;    thymidine kinase;    BudR;    5-bromodeoxyuridine;   

DOI  :  10.1016/0014-5793(89)80759-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A human thyroid peroxidase cDNA, hTPO-1 [(1987) Proc. Natl. Acad. Sci. USA 84, 5555–5559], was expressed in human Hep G2 cells using a vaccinia virus cDNA-expression system. When examined by immunoblot analysis, the level of hTPO-1 protein expression reached a maximum approx. 24 h after infection and remained at a similar level up to 72 h post-infection. The expressed protein was enzymatically active as measured by guaiacol oxidation. Monoclonal antibody-assisted immunoaffinity column chromatography was used for partial purification of vaccinia-expressed hTPO-1, resulting in more than 300-fold higher specific activity and a measurable difference spectrum of the hTPO-1 (Fe³⁺)-CN complex.

【 授权许可】

Unknown