Topography of RNA in the ribosome: location of the 5 S RNA residues A39 and U40 on the central protuberance of the 50 S subunit

FEBS Letters

Vasiliev, V.D.¹  Evstafieva, A.G.¹  Bogdanov, A.A.¹  Shatsky, I.N.¹ 

[1] A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, 117234 MoscowUSSR

关键词: Ribosome;    5 S RNA;    Nuclease S1;    Dinitrophenyl hapten;    Immune electron microscopy;   

DOI  :  10.1016/0014-5793(85)80740-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The internal site of 5 S RNA comprising residues A₃₉ and U₄₀ has been localized on the E.coli 50 S ribosomal subunit by immune electron microscopy. It has been found to be located on the interface side of the central protuberance at the position distinctly apart but very close to the position of the 5 S RNA 3'-end providing evidence for a quite compact folded conformation of the 5 S RNA in situ.

【 授权许可】

Unknown