1α,25‐Dihydroxy vitamin D3 (calcitriol) stimulates proliferation of human circulating monocytes in vitro

FEBS Letters

Ozawa, Keiya¹  Okabe, Tetsuro¹  Urabe, Akio¹  Ohta, Masataka¹  Takaku, Fumimaro¹ 

[1] The Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Hongo 7-3-1, Tokyo 113, Japan

关键词: Iα;    25-Dihydroxy vitamin D3;    Calcitriol;    Vitamin D3;    Monocyte;    Macrophage;    Monocyte-growth factor;    1;    25(OH)2D3;    1α;    25-dihydroxyvitamin D3;    1(OH)D3;    1α-hydroxyvitamin D3;    25(OH)D3;    25-hydroxyvitamin D3;    1;    24(OH)2D3;    1α;    24R-dihydroxyvitamin D3;    1;    24;    25(OH)3D3;    1α-;    24R;    25-trihydroxyvitamin D3;    MoGF;    monocyte-growth factor;    PHA;    phytohemagglutinin antigen;    PHA-LCM;    PHA-stimulated lymphocyte-conditioned medium;   

DOI  :  10.1016/0014-5793(85)80730-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previous studies demonstrated that human circulating monocytes can proliferate in vitro when incubated with lectin-induced factor(s) from lymphocytes [(1985) Biochem. Biophys. Res. Commun., in press]. This study shows that human monocytes were induced to proliferate when incubated with 1α,25-dihydroxy vitamin D₃ (calcitriol) at physiological concentrations. The optimal dose was about 10 nM. Proliferative activity was examined both by measuring the [su3H]thymidine incorporation and by counting cell nuclei. Among other derivatives of vitamin D₃, 1α,24 R-dihydroxyvitamin D₃ and 1α,24R,25-trihydroxyvitamin D₃ stimulated mitotic activity of monocytes. Addition of both calcitriol and lectin-stimulated lymphocyte-conditioned medium to the monocyte culture had an additional effect on the mitotic activity of monocytes.

【 授权许可】

Unknown