Mechanism of activation of H2O2 by peroxidases: kinetic studies on a model system

FEBS Letters

Pratt, John M.¹  Baldwin, David A.¹  Marques, Helder M.¹ 

[1] Department of Chemistry, University of the Witwatersrand, Jan Smuts Avenue, Johannesburg 2001, South Africa

关键词: Peroxidase;    Proton-coupled reaction;    Arginine;    Microperoxidase-8;    Hydrogen;    peroxide;    Guanidinium;    Gc;    guiacol;    GuaH+;    guanidinium ion;    MP-8;    microperoxidase-8;   

DOI  :  10.1016/0014-5793(85)80799-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Kinetic studies on the peroxidase activity of microperoxidase-8 at pH 5.5–8.5 show that the rate is increased by raising the pH or by the presence of guanidinium ion. Comparison with published data on the peroxidases provides evidence that the enzyme activates H₂O₂ through the cooperative binding of H⁺ + HO⁻ ₂ and suggests a role for the invariant distal Arg.

【 授权许可】

Unknown