Convenient modification of the method for oligonucleotide‐directed in vitro mutagenesis of cloned DNA

FEBS Letters

Efimov, V.A.¹  Chakhmakhcheva, O.G.¹  Mirskikh, O.V.¹  Ovchinnikov, Yu.A.¹ 

[1] Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Ul. Miklukho-Maklaya, 16/10, Moscow 117871, USSR

关键词: Recombinant DNA;    Oligonucleotide-directed mutagenesis;    Colony screening;   

DOI  :  10.1016/0014-5793(85)80302-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new modification of the oligonucleotide-mediated mutagenesis technique has been developed. The proposed methodology has been used to produce specific base changes in the double-stranded plasmid DNA. For this purpose, special cloning vectors have been constructed using the synthetic oligodeoxyribonucleotides. The developed method allows the production of mutant DNA from those of the wild-type with a yield of 10–20%.

【 授权许可】

Unknown