Mutagenesis directed by phosphotriester analogues of oligonucleotides: A way to site‐specific mutagenesis in vivo

FEBS Letters

Petrenko, V.A.¹  Boldyrev, A.N.¹  Kipriyanov, S.M.¹  Pozdnyakov, P.I.¹ 

[1] All-Union Institute of Molecular Biology, Kol'tsovo, Novosibirsk Region 633159, USSR

关键词: Oligonucleotide-directed mutagenesis;    Phage M13;    β-Galactosidase;    DNA sequencing;    Mutagenesis;    single-stranded DNA;   

DOI  :  10.1016/0014-5793(88)80236-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new approach to induce directed mutations in genes of study through simple cotransfection of E. coli cells by the mixture of primer and template was developed. This method is based on the use of synthetic phosphotriester analogues of oligonucleotides as site-specific mutagenic primers. The achieved yield of mutant clones was 2–3%.

【 授权许可】

Unknown