Identification of a rat liver cDNA and mRNA coding for the 28 kDa gap junction protein

FEBS Letters

Traub, Otto¹  Willecke, Klaus¹  Heynkes, Roland¹  Kozjek, Gisela¹ 

[1] Institut für Zellbiologie (Tumorforschung), Universität Essen, Hufelandstr. 55, 43 Essen 1, FRG

关键词: Colony screening;    Deoxyinosine oligonucleotide;    Gap junction;    cDNA;    Northern blot;    Hepatocyte;    (Mouse);   

DOI  :  10.1016/0014-5793(86)80865-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By screening of a rat liver cDNA library with complex and deoxyinosine containing oligonucleotide probes a cDNA clone was isolated and shown by sequencing to code for the amino-terminal half of the rat liver 28 kDa gap junction protein. The insert hybridized to a 1.9 kb species from rat and mouse liver poly(A)⁺ RNA in Northern blot analysis. In embryonic mouse hepatocytes the amount of the 1.9 kb mRNA increased 3-fold between 24 and 96 h in culture. This correlates with the previously described increase of the 28 kDa gap junction protein under these conditions.

【 授权许可】

Unknown