Immunological properties of the dimeric and tetrameric hemoglobins from the mollusc Scapharca inaequivalvis

FEBS Letters

Citro, Gennaro¹  Chiancone, Emilia²  Verzili, Daniela²  Ascoli, Franca³ 

[1] Regina Elena Institute for Cancer Research, 00161 RomeItaly;CNR Center of Molecular Biology, Institutes of Chemistry and Biochemistry, Faculty of Medicine, University of Rome ‘La Sapienza’, Piazzale Aldo Moro 5, 00185 RomeItaly;Department of Cell Biology, University of Camerino, 62032 Camerino (Mc, Italy

关键词: Molluscan hemoglobin;    Antibody;    Immunological cross-reactivity;   

DOI  :  10.1016/0014-5793(85)80290-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The dimeric and tetrameric hemoglobins from the bivalve mollusc Scapharca inaequivalvis produce highly specific antibodies in rabbits. No antigenic cross-reactivity between the two hemoglobins has been observed despite the presence of highly conserved regions, i.e., the E and F helices, in the dimer and in the α-chain of the tetramer. These results may be explained on the basis of the unusual assembly of S. inaequivalvis hemoglobins, in which the E and F helices are not exposed to solvent as in vertebrate hemoglobins, but form a crucial intersubunit contact. The specific antibodies only slightly affect the oxygen binding properties of the respective antigen.

【 授权许可】

Unknown