An electrogenic uniport mediates light‐dependent Ca2+ influx into intact spinach chloroplasts

FEBS Letters

Kreimer, Georg¹  Melkonian, Michael¹  Latzko, Erwin¹ 

[1] Botanisches Institut der Universität Münster, Schloßgarten 3, D-4400 Münster, FRG

关键词: Spinach;    Chloroplast;    Ca2+;    Electrogenic uptake;    Uniport;    CCCP;    carbonyl cyanide m-chlorophenyl hydrazone;    FCCP;    carbonyl cyanide p-trifluorome-thoxyphenylhydrazone;    DCMU;    3-(3;    4-dichlorophenyl)-1;    1-dimethylurea;    ΔμH+ proton motive force;    ΔE;    membrane potential;    ΔpH;    transmembrane pH gradient;    PGA;    3-phosphoglyceric acid;   

DOI  :  10.1016/0014-5793(85)81081-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Light-dependent Ca²⁺ influx into intact spinach chloroplasts, measured with the metallochromic indicator arsenazo III, is stimulated by uncouplers (FCCP, CCCP, nigericin) and inhibited by ruthenium red. The data presented demonstrate that light-dependent Ca²⁺ influx into chloroplasts is electrogenic and mediated by a uniport-type carrier. The characteristics of the carrier system are similar to those of the Ca²⁺ uniport of mitochondria.

【 授权许可】

Unknown