Cyclic AMP stimulates dephosphorylation of specific proteins in intact S49 mouse lymphoma cells

FEBS Letters

Kiss, Zoltán²  Steinberg, Robert A.¹ 

[1] Biological Sciences Group, U-44, The University of Connecticut, Storrs, CT 06268, USA;Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, 6701 Szeged, Hungary

关键词: Cyclic AMP;    Dephosphorylation;    Protein kinase;    Protein phosphatase;    Two-dimensional gel;    cAMP;    cyclic AMP;    DME;    Dulbecco's modified Eagle's medium;   

DOI  :  10.1016/0014-5793(85)81072-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two-dimensional gel electrophoresis of proteins labeled with ³²P₁ in S49 mouse lymphoma cells revealed five phosphoproteins that were rapidly and reversibly dephosphorylated in response to elevation of cyclic AMP (cAMP). Under basal conditions, labeling of at least two of these proteins was limited by slow turnover of protein-bound phosphate. The rapid cAMP-mediated dephosphorylation of these species was attributable, therefore, to stimulation of a specific phosphoprotein phosphatase.

【 授权许可】

Unknown