Purification of rabbit tumor necrosis factor

FEBS Letters

Yamazaki, Masatoshi²  Abe, Shigeru²  Gatanaga, Tetsuya²  Soma, Gen'ichiro¹  Mizuno, Den'ichi² 

[1] Meiji College of Pharmacy, Tanashi, Tokyo 188, Japan;Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Tsukui, Kanagawa 199-01, Japan

关键词: Tumor necrosis factor;    Purification;    Amino acid sequence;    TNF;    tumor necrosis factor;   

DOI  :  10.1016/0014-5793(85)81071-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rabbit tumor necrosis factor (TNF) was purified and shown by SDS-PAGE to be a single protein of 18 kDa. TNF in 355 ml rabbit serum was precipitated with ammonium sulfate, and purified by repeated DEAE-Sephadex and Sephacryl S-200 chromatographies, and the final fractionation on Blue-Sepharose 6B. By this procedure its yield was 22% and its specific activity was 2.4 × 10⁷ U/mg protein. The sequence of the N-terminal 20 amino acids was determined.

【 授权许可】

Unknown