Mononuclear cell supernatants inhibit prolyl hydroxylation

FEBS Letters

Meyers, D.F.¹  Page, R.C.¹  Narayanan, A.S.¹ 

[1] Departments of Pathology and Periodontics, University of Washington School of Medicine, Seattle W98195, USA

关键词: Collagen synthesis;    Lymphokine;    Lymphocyte supernatant;    Prolyl hydroxylation;    Fibroblast;   

DOI  :  10.1016/0014-5793(85)80524-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of phytohemagglutinin(PHA)-activated human peripheral mononuclear cell supernatant (AS) on collagen production by human fibroblasts was examined. The AS inhibited collagen production in a doseand time-dependent manner. Labeling and pulse chase experiments showed that it did not block collagen secretion, but a greater proportion of molecules synthesized in its presence accumulated within the cells. Amino acid analysis showed that when labeling was done at 24°C prolyl hydroxylation in fibroblasts exposed to the AS was reduced to two-thirds of the cultures treated with control supernatant (CS), however it was not different at 37°C. These results indicate that the AS inhibits collagen hydroxylation, that the un(under)hydroxylated collagen molecules are degraded at physiological temperature and that suppression of collagen hydroxylation may be a mechanism by which the AS inhibits collagen production.

【 授权许可】

Unknown