Double‐quantum 1H NMR studies of nitrogen metabolism in yeast

FEBS Letters

Paul Juretschke, Hans¹ 

[1] Institut für Biophysikalische Chemie, Johann-Wolfgang-Goethe Universität, Theodor-Stern-Kai 7, D-6000 Frankfurt am Main 70, FRG

关键词: 1H-NMR;    In vivo NMR;    Nitrogen metabolism;    Yeast metabolism;    2D NMR;   

DOI  :  10.1016/0014-5793(84)81254-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using double-quantum ¹H NMR spectroscopy, the nitrogen metabolism of yeast cells pulse-labelled with ¹⁵N-enriched ammonium sulfate has been detected. The sensitivity of this indirect method is far higher than direct observation of the ¹⁵N signals by ¹⁵N NMR. Hence, a spectrum with an excellent signal-to-noise ratio was obtained in 25.6 s. The observed proton signals were assigned to specific metabolites using two-dimensional reverse shift correlation spectra.

【 授权许可】

Unknown