15N‐NMR studies of nitrogen assimilation and amino acid biosynthesis in the ectomycorrhizal fungus Cenococcum graniforme

FEBS Letters

Martin, Francis¹ 

[1] Laboratoire de Microbiologie Forestière, Institut National de la Recherche Agronomique. Centre de Recherche Forestières de Nancy, Champenoux 54280 Seichamps, France

关键词: 15N-NMR;    In vivo NMR;    Nitrogen metabolism;    Amino acid;    Ectomycorrhizal fungus;   

DOI  :  10.1016/0014-5793(85)80331-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

¹⁵N-NMR spectroscopy was used to follow nitrogen assimilation and amino acid biosynthesis in the intact mycelium and extracts of the symbiotic fungus Cenococcum graniforme. The primary incorporation of ¹⁵NH₄ ⁺ appears to proceed via the glutamine synthetase activity. Glutamine, γ-aminobutyrate, alanine and arginine were the main labelled nitrogenous pools. The quenching of arginine in intact mycelium suggest that the amino acid is associated with a cellular component which restricts its rotational freedom.

【 授权许可】

Unknown