In vivo proton NMR spectroscopy

FEBS Letters

Kathryn M, Nicholls¹  Doddrell, David M.²  Sanders, Jeremy K.M.¹ 

[1] University Chemical Laboratory, Lensfield Road, Cambridge, CB2 1EW, England;School of Science, Griffith University, Nathan 4111, Australia

关键词: 1H NMR;    In vivo NMR;    Formaldehyde;    Metabolism;    Detoxification;    E. coli;   

DOI  :  10.1016/0014-5793(84)81371-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The metabolism of [¹³C]formaldehyde in E.coli has been observed by proton NMR; the reverse-POMMIE pulse sequence, which saturates all ¹H signals and then transfer magnetisation from ¹³C to ¹H, suppresses water by 10⁵-fold, allowing observation of metabolism at the millimolar level

【 授权许可】

Unknown