Specific interactions of liposomes with PMN leukocytes upon incorporating tuftsin in their bilayers

FEBS Letters

Singhal, Arun¹  Gupta, C.M.¹  Bali, Anu¹  Jain, Rakesh K.¹ 

[1] Division of Biophysics, Central Drug Research Institute, Lucknow 226 001, India

关键词: Drug targeting;    Liposome;    Ligand-receptor interaction;    Tuftsin;    Leukocyte;    Endocytosis;    PC;    phosphatidylcholine;    CH;    cholesterol;    6-CF;    6-carboxyfluorescein;    TNBS;    trinitrobenzenesulfonic acid;    TBS (mM);    Tris-HCl (10);    NaCl (150);    pH 7.4;    HBSS (mM);    NaCl (137);    KCl (5.37);    CaCl2·2H2O (1.00);    MgSO4·7H2O (0.41);    MgCl2·6H2O (0.50);    KH2PO4 (0.44);    Na2HPO4·2H2O (0.34);    glucose (5.55);    NaHCO3 (4.16);    pH 7.4;    SSTS (mM);    Tris-HCl (10);    NaCl (150);    sucrose (44);    EDTA (5);    pH 7.4;   

DOI  :  10.1016/0014-5793(84)81251-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Incorporation of tuftsin derivatives, Thr-Lys-Pro-Arg-NH-C₁₈H₃₇ or Thr-Lys-Pro-Arg-NH-(CH₂)₂-NH-COC₁₅H₃₁, into an egg phosphatidylcholine/cholesterol liposome bilayer led to significantly enhanced binding of the liposomes to PMN leukocytes at 37°C but not at 0°C. Under identical conditions, no such enhanced binding of the liposomes was observed with erythrocytes and lymphocytes. These results demon-strate that grafting of tuftsin on the liposome bilayer enables the liposome to recognize specifically the PMN leukocytes and to deliver its contents to these cells.

【 授权许可】

Unknown