Antibody‐coated liposomes

FEBS Letters

Senior, Judith²  Gregoriadis, Gregory²  Waters, J.A.¹ 

[1] Immunology Group, Academic Department of Medicine, Royal Free Hospital School of Medicine, London NW3 2QC, England;Medical Research Council Group, Academic Department of Medicine, Royal Free Hospital School of Medicine, London NW3 2QC, England

关键词: Liposome;    Drug targeting;    Antibody;    Monoclonal anti-HBSAg IgG1;    Liposomal stability;   

DOI  :  10.1016/0014-5793(86)80213-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Incubation of small unilamellar liposomes composed ofequimolar phospholipid and cholesterol with mouse IgG or mouse monoclonal IgG₁ for up to 24 h resulted in considerable (34-89%) adsorption of the protein onto the liposomal surface. Immunoglobulin remained adsorbed after exposure to mouse plasma and, in the case of monoclonal (anti-HBSAg) IgG₁, was able to mediate association of the liposomes with the antigen. Extensive immunoglobulin adsorption suggests caution in interpreting co valent linkage values obtained upon prolonged incubation of chemically modified antibodies with liposomes. It may also be a preferred alternative in preparring targeted liposomes for intravenous use when chemically modified antibodies promote premature clearance of the antibody-liposome complex from the circulation.

【 授权许可】

Unknown