Chemiluminescent assay of lipid peroxide in plasma using cytochrome c heme peptide

FEBS Letters

Tabata, Fumiko¹  Iwaoka, Teiki¹ 

[1] Analytical and Metabolic Research Laboratories, Sankyo Co. Ltd., 1-2-58, Hiromachi, Shinagawa-ku, Tokyo, Japan 140

关键词: Chemiluminescent assay;    Luminol sensitization;    Cytochrome c heme peptide;    Lipid hydroperoxide;    Thiobarbituric acid assay;    In vivo lipid peroxidation;   

DOI  :  10.1016/0014-5793(84)81237-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A convenient assay specific to lipid hydroperoxide in plasma is presented. Cytochrome c heme peptide obtained from Saccharomyces was found to emit a strong chemiluminescence with any hydroperoxide, but not with TBA-reactive substances. The benefit of measuring this luminescence using photon counting is discussed with respect to in vivo lipid peroxidation.

【 授权许可】

Unknown