Site‐specific cleavage of double‐strand DNA by hydroperoxide of linoleic acid

FEBS Letters

Inouye, Satoshi¹ 

[1] Department of Biochemistry, Kyushu University 60 School of Medicine, Fukuoka 812, Japan

关键词: Lipid hydroperoxide;    Site-specific DNA cleavage;    Hydroperoxylinoleic acid;    Unsaturated fatty acid;    Lipoxy genase;    LAHPO;    13-L-hydroperoxy-cis-9;    trans-11-octadecadienoic acid;    LAHO;    13-L-hydroxy-cis-9;    trans-11-octadecadienoic acid;    LA;    linoleic acid;    dsDNA;    double-strand DNA;   

DOI  :  10.1016/0014-5793(84)81131-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The breakage of double-strand (ds) DNA by 13-L-hydroperoxy-cis-9, trans-11-octadecadienoic acid (LAHPO) was investigated by agarose gel electrophoresis of supercoiled pBR322 DNA and the site of cleavage on the DNA molecule was determined by the method of DNA sequence analysis using 3'-end and 5'-end-labeled DNA fragments as substrates. LAHPO caused cleavage at the position of guanine nucleotide in dsDNA. LAHPO caused dsDNA breaks at specific sites, but linoleic acid (LA) and 13-L-hydoxy-cis-9,trans-11-octadecadienoic acid (LAHO) have no such effects on dsDNA. The active oxygen atom of the hydroperoxy group of LAHPO was perhaps responsible for the site-specific cleavage of dsDNA.

【 授权许可】

Unknown