Glycogen metabolism: a 13C‐NMR study on the isolated perfused rat heart

FEBS Letters

Lavanchy, N.¹  Rossi, A.¹  Martin, J.² 

[1] Laboratoire de Physiopathologie du Métabolisme Cardiaque, CNRS UA 632 USMG, BP 68, 38402 St Martin d'Heres CédexFrance;Centre d'Etudes Nucléaires de Grenoble, Grenoble, France

关键词: Perfused rat heart;    13C-NMR;    D-[1-13C]Glucose;    Glycogen metabolism;   

DOI  :  10.1016/0014-5793(84)81234-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glycogen synthesis from D-[1-¹³C]glucose was observed in the perfused rat heart by ¹³C-NMR spectroscopy at 62.9 MHz. The glycogenogenesis was stimulated by pretreatment of the animals with isoprenaline. Whereas in hearts from control rats the incorporation of D-[1-¹³C]glucose into the glycogen remained below the detection threshold, 5 min proton-decoupled ¹³C-NMR spectra revealed, in hearts from treated rats, a significant labelling of the glycogen within the first minutes of the perfusion and a further linear increase of the glycogen resonance for up to 25 min. This model was used to monitor the appearance of ¹³C-labelled lactate during ischemia.

【 授权许可】

Unknown