FEBS Letters | |
High resolution proton NMR studies of perfused rat hearts | |
Cohn, Jay N.2  Michurski, Steve1  Maidan, Rubin2  Ugurbil, Kamil1  Petein, Marc2  From, Arthur H.2  | |
[1] Department of Biochemistry and the Gray Freshwater Biological Institute, University of Minnesota, Navarre, MN 555392, USA;Cardiovascular Division, Department of Medicine, Minneapolis VA Medical Center and University of Minnesota Medical School, Minneapolis, MN 55455, USA | |
关键词: 1H-NMR; Perfused rat heart; Myocardial metabolism; | |
DOI : 10.1016/0014-5793(84)80835-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
High resolution 1H NMR spectra of perfused rat hearts have been obtained under normoxic, ischemic and hypoxic conditions. Several myocardial metabolites including taurine, carnitine, lactate and tissue glycerides are detected in the 1H NMR spectra. Changes in oxygen availability induce perturbations in the levels of some metabolites, in particular, lactate. Experiments with fasted rats and with substrate-free perfusion suggest that the glycerides detected in 1H spectra are metabolically mobilizable but have a slow rate of turnover. These results demonstrate that utility of 1H NMR in monitoring myocardial metabolism.
【 授权许可】
Unknown
【 预 览 】
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RO201912020285202ZK.pdf | 563KB | download |