NMR studies on oligo‐deoxyribonucleotides containing N 6‐methyladenine

FEBS Letters

Guschlbauer, Wilhelm¹  Téoule, Robert¹  Fazakerley, G.Victor¹  Guy, André² 

[1] Service de Biochimie, Bât. 142, Département de Biologie, Centre d'Etudes Nucléaires de Saclay, F-91191 Gif-Sur-Yvette CedexFrance;Laboratoire de Chimie, Département de Recherche Fondamentale, Centre d'Etudes Nucléaires de Grenoble, 85X, F-38041 Grenoble Cedex, France

关键词: Synthetic oligonucleotide;    NOE;    Recognition site;    dam methylase;   

DOI  :  10.1016/0014-5793(84)81216-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

500 MHz ¹H-NMR and NOE measurements of d(GGATCC) and d(GGm⁶ATCC) show that both oligo-nucleotides assume a B-DNA conformation at low temperature. Around the melting temperature, however, the single and double strands of the N-methylated form are in slow exchange on the NMR time scale. The preferred conformation of the adenine methyl group, cis to N¹, retards base pairing and also destabilizes the double helix.

【 授权许可】

Unknown