Does the DNA methylase Eco dam pair nucleotide sequences to form site‐specific duplexes?

FEBS Letters

Gorbunov, Yu.A.²  Buryanov, Ya.I.³  Nesterenko, V.F.³  Vienozhinskis, M.T.¹  Malygin, E.G.²  Popov, S.G.²  Zinoviev, V.V.² 

[1] Institute of Biochemistry, Lithuanian SSR Academy of Sciences, Vilnius 232021, USSR;All-Union Research Institute of Molecular Biology, Novosibirsk Region, Koltsovo USSR;Institute of Biochemistry and Physiology of Microorganisms, USSR Academy of Sciences, Pushchino, Moscow Region 142292, USSR

关键词: DNA methylase;    Single-stranded DNA sequence;    Recognition site;    Methylatable substrate structure;   

DOI  :  10.1016/0014-5793(84)80228-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Eco dam methylase is active on denatured DNA and single-stranded synthetic oligonucleotides containing GATC sites. The results suggest that on interaction with single-stranded oligonucleotides the Eco dam methylase is able to form a duplex structure within the GATC site, and that this duplex site is a substrate for enzyme.

【 授权许可】

Unknown