Internal mobility in a double‐stranded B DNA hexamer and undecamer

FEBS Letters

Clore, G.Marius¹  Gronenborn, Angela M.¹ 

[1] Division of Physical Biochemistry, National Institute for Medical Research, Mill Hill, London NW7 1AA, England

关键词: B DNA solution dynamics;    Synthetic oligonucleotide;    1H-1H NOE;    NOE;    nuclear Overhauser enhancement;   

DOI  :  10.1016/0014-5793(84)81129-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The internal mobility of the deoxyribose H2'-H2' ' and base C(H5)-C(H6) and T(CH₃)-T(H6) vectors has been investigated by means of time-dependent nuclear Overhauser enhancement (NOE) measurements in a B DNA hexamer and undecamer. Cross-relaxation rates between these proton pairs are determined from the initial slopes of the time development of the NOEs, and, as the interproton distances between these proton pairs are fixed, apparent correlation times for the 3 interproton vectors are calculated from the cross-relaxation rate data. It is shown that there is little residue to residue variation in the crossrelaxation rates of the interproton vectors within each oligonucleotide, that the mean apparent correlation times of the C(H5)-C(H6) and T(CH3)-T(H6) vectors are approximately equal and significantly greater than that of the H2'-H2' ' vectors, and that the data for the H2'-H2' ' vectors of both oligonucleotides and the C(H5)-C(H6) and T(CH3)-T(H6) vectors of the undecamer cannot be accounted for by isotropic tumbling alone. The data are analysed in terms of a two motion model with isotropic tumbling and a single internal motion. The relaxation time of the internal motion at 23 °C is ≲ 1 ns for the H2'-H2' ' vectors of both oligonucleotides and ≲3 ns for the C(H5)-C(H6) and T(CH₃)-T(H6) vectors of the undecamer. In the case of the H2'-H2' ' vectors, however, the amplitude of the internal motion is found to be too large to be compatible with the known stereochemistry of DNA. This finding can only be explained by invoking additional degrees of internal freedom with a larger number of internal motions of small amplitude of the type deduced from the analysis of crystallographic thermal factors [(1984) J. Mol. Biol. 173, 361-388].

【 授权许可】

Unknown