Modification of the amino acid acceptor stem of E. coli tRNAf Met by ligation of chemically synthesized ribooligonucleotides

FEBS Letters

Doi, Takefumi¹  Morioka, Hiroshi¹  Matsugi, Jitsuhiro¹  Ikehara, Mono¹  Ohtsuka, Eiko¹ 

[1] Faculty of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565, Japan

关键词: Aminoacylation;    Formylation;    Elongated aminoacylation end;    Truncated aminoacylation end ligation;    Synthetic oligonucleotide;   

DOI  :  10.1016/0014-5793(85)80441-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The single-stranged region of the amino acid acceptor stem corresponding to the 3'-end of E. coli tRNA^Met _f was replaced by ligation of chemically synthesized ribooligonucleotides, in order to change the length of the single-stranded CCA terminus. The chemically synthesized ribooligomers, CCA, ACCA, AACCA and CAACCA, were ligated to nuclease-treated E. coli tRNA^Met _f, which lacked the ACCA sequence at the 3'-end. The methionine acceptor activities of these modified tRNAs were examined using E. coli methionyl-tRNA synthetase. Ligation of the chemically synthesized pentamer (AACCA) to the acceptor terminus restored the methionine acceptor activity, whereas ligation of the hexamer (CAACCA) or trimer (CCA) to the acceptor terminus did not Modification of the acceptor terminus had no effect on the formylation of accepted methionine.

【 授权许可】

Unknown