A new approach for using cofactor dependent enzymes: example of alcohol dehydrogenase

FEBS Letters

Sicsic, Sames¹  le Goffic, François¹  Langrene, Sylvie¹  Durand, Philippe¹ 

[1] Centre d'Etudes et de Recherches en Chimie Organique Appliquée. CNRS, BP 28, F-94320 Thiais, France

关键词: NAD(H);    NMN(H);    Nicotinamide mononucleoside;    Alcohol dehydrogenase;    Enzymatic oxidation;    Enzymatic reduction;   

DOI  :  10.1016/0014-5793(84)81188-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The use of enzymes requiring a cofactor as substrate in organic synthesis is still a problem since the cofactors are expensive. This study deals with a new approach consisting of using fragments of NAD⁺. Three fragments of NAD(H) are examined. The activities of NMN⁺ and NMNH are greatly improved by the addition of adenosine in ethanol oxidation and in cyclohexanone reduction, respectively. Nicotinamide monocucleoside is not active in the ethanol oxidation but the addition of AMP promotes this reaction.

【 授权许可】

Unknown