Platelet‐activating factor stimulates phospholipase in quiescent Swiss mouse 3T3 fibroblast

FEBS Letters

Yasuda, Hisakazu¹  Kawaguchi, Hideaki¹ 

[1] Department of Cardiovascular Medicine, Hokkaido University School of Medicine, Sapporo 060, Japan

关键词: Platelet-activating factor;    Phospholipase A2;    Phospholipase C;    Arachidonic acid;    Diacylglycerol;    PAF;    platelet-activating factor;    DMEM;    Dulbecco's modified Eagle's medium;    PLase;    phospholipase;    PL;    phospholipids;    Lyso-PC;    lysophosphatidylcholine;    PC;    phosphatidylcholine;    PI;    phosphatidylinositol;    PE;    phosphatidylethanolamine;    MG;    monoacylglycerol;    DG;    diacylglycerol;    TG;    triacylglycerol;    TLC;    thin layer chromatography;   

DOI  :  10.1016/0014-5793(84)80918-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Platelet-activating factor(PAF) induced the rapid hydrolysis of phospholipids in Swiss mouse 3T3 fibroblasts prelabeled with [³H]arachidonic acid or [³H]choline. Up to 23% of the [³H]arachidonic acid incorporated in phospholipids was released into the medium when PAF was added to cells. We also observed increases of diacylglycerol and lysophosphatidylcholine in the medium and cells after the addition of PAF. These results support the possibility that platelet-activating factor stimulates phospholipase A₂ and C activities in 3T3 cells.

【 授权许可】

Unknown