Identification of a membrane‐associated 1‐0‐alkyl‐2‐arachidonoyl‐sn‐glycero‐3‐phosphocholine hydrolyzing phospholipase A2in guinea pig 1 epidermis Implications in the cutaneous biosynthesis of platelet‐activating factor

FEBS Letters

Tang, Wilson¹  Wong, Barnett¹  Ziboh, Vincent A.¹ 

[1] Department of Dermatology, School of Medicine, University of Califorina, Davis CA 95616, USA

关键词: Phospholipase A2;    Platelet-activating factor;    Membrane enzyme;    Epidermis;   

DOI  :  10.1016/0014-5793(92)80670-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A membrane-associated 1-0-alkyl-2-arachidonoyl-GPC hydrolyzing phospholipase A₂ was identified in guinea pig epidermis. It is regio-speciflc (associated with the particulate microsomal fraction) and specific for the hydrolysis of 1-0-alkyl-2-arachidonoyl-sn-glycero-3-phosphocholine. It is sensitive to low calcium concentrations suggesting that it may be activated by increasirg intracellular calcium. Since ether-linked phospholipids are known to exist in the epidermis, further understanding of the properties of this 1-0-alkyl-arachidonoyl-hydrolyzing PLA₂ may allow us to control the generation of 1-0-alkyl-2-lyso-sn-glycero-3-phosphocholine, a key substrate for the generation or the platelet-activating factor in the tissue.

【 授权许可】

Unknown