期刊论文详细信息
FEBS Letters | |
Interactions between cro repressor and the model specific binding site | |
Chernov, B.K.1  Kurochkin, A.V.1  Skryabin, K.G.1  Kirpichnikov, M.P.1  | |
[1] Institute of Molecular Biology, The USSR Academy of Sciences, V-334, Vavilov Str. 32, 117984 Moscow, USSR | |
关键词: cro repressor; Protein structure; Operator; Specific binding; 1H NMR; NMR; nuclear magnetic resonance; HPLC; high pressure liquid chromatography; DSS; sodium 2; 2-dimethyl-2-silapentane-5-sulphonate; CD; circular dichroism; NOE; nuclear Overhauser enhancement; | |
DOI : 10.1016/0014-5793(84)80759-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Binding of λ phage cro repressor to the synthetic half of OR3, the most conservative half of the specific binding sites, was investigated by proton nuclear magnetic resonance spectroscopy. It was found that the α-helical segment (27–36) of the protein was involved in specific interactions with the model binding site. The 3-dimensional structure of cro repressor does not change noticeably upon complex formation. Intercalation can be excluded as a possible means of interaction.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020285898ZK.pdf | 337KB | download |