FEBS Letters | |
PHI‐like immunoreactivity in the gallbladder and in vitro effect of porcine PHI on smooth muscle of the gallbladder | |
Polak, J.M.2  Bloom, S.R.1  Piper, P.J.3  Yiangou, Y.1  Gu, J.2  Christofides, N.D.1  | |
[1] Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 OHS England;Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 OHS England;Departments of Medicine and Department of Pharmacology, Royal College of Surgeons, Lincoln's Inn Fields, London WC2, England | |
关键词: PHI; VIP; Gallbladder; Chromatography; Pharmacology; | |
DOI : 10.1016/0014-5793(84)80757-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The occurrence and distribution of PHI-like immunoreactivity in the guinea pig gallbladder has been analysed by radioimmunoassay and immunocytochemistry. Chromatography of gallbladder extracts by gel permeation and high-performance liquid chromatography revealed that guinea pig PHI-like immunoreactivity is of a similar size to that of porcine PHI but may differ in its amino acid sequence. Immunocytochemistry showed PHI-immunoreactivity to be localised to nerves found predominantly in the ganglionated plexus and themucosal plexus of the gallbladder. Pure natural porcine PHI induced a dose-dependent relaxation of the isolated guinea pig gallbladder muscle which was not blocked by antagonists to acetylcholine, catecholamines, histamine, and 5-hydroxytryptamine. PHI may thus be one of the local factors involved in controlling gallbladder function.
【 授权许可】
Unknown
【 预 览 】
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