PHI‐like immunoreactivity in the gallbladder and in vitro effect of porcine PHI on smooth muscle of the gallbladder

FEBS Letters

Polak, J.M.²  Bloom, S.R.¹  Piper, P.J.³  Yiangou, Y.¹  Gu, J.²  Christofides, N.D.¹ 

[1] Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 OHS England;Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 OHS England;Departments of Medicine and Department of Pharmacology, Royal College of Surgeons, Lincoln's Inn Fields, London WC2, England

关键词: PHI;    VIP;    Gallbladder;    Chromatography;    Pharmacology;   

DOI  :  10.1016/0014-5793(84)80757-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The occurrence and distribution of PHI-like immunoreactivity in the guinea pig gallbladder has been analysed by radioimmunoassay and immunocytochemistry. Chromatography of gallbladder extracts by gel permeation and high-performance liquid chromatography revealed that guinea pig PHI-like immunoreactivity is of a similar size to that of porcine PHI but may differ in its amino acid sequence. Immunocytochemistry showed PHI-immunoreactivity to be localised to nerves found predominantly in the ganglionated plexus and themucosal plexus of the gallbladder. Pure natural porcine PHI induced a dose-dependent relaxation of the isolated guinea pig gallbladder muscle which was not blocked by antagonists to acetylcholine, catecholamines, histamine, and 5-hydroxytryptamine. PHI may thus be one of the local factors involved in controlling gallbladder function.

【 授权许可】

Unknown