The VIP2 receptor: Molecular characterisation of a cDNA encoding a novel receptor for vasoactive intestinal peptide

FEBS Letters

Lutz, E.M.¹  Sheward, W.J.¹  Fink, G.¹  Morrow, J.A.¹  West, K.M.¹  Harmar, A.J.¹ 

[1] MRC Brain Metabolism Unit, Department of Pharmacology, University of Edinburgh, 1 George Square, Edinburgh, EH8 9J2, UK

关键词: Vasoactive intestinal peptide (VIP);    G protein-linked receptor;    Rat pituitary;    Rat olfactory bulb;    cDNA cloning;    cAMP;    adenosine 3′:5′-cyclic monophosphate;    cDNA;    complementary DNA;    CGRP;    calcitonin gene related peptide;    CRF;    corticotrophin releasing factor;    dNTP;    deoxynucleotide triphosphate;    DMEM;    Dulbecco's modified Eagle's medium;    rGHRH;    rat growth hormone releasing hormone;    GLP;    glucagon-like peptide I;    mRNA;    messenger RNA;    PACAP;    pituitary adenylate cyclase activating polypeptide;    PCR;    polymerase chain reaction;    PHI;    peptide histidine isoleucine;    PTH;    parathyroid hormone;    RNase;    ribonuclease;    VIP;    vasoactive intestinal peptide;   

DOI  :  10.1016/0014-5793(93)81668-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned and sequenced a cDNA (RPR4) encoding a new member of the secretin/calcitonin/parathyroid hormone (PTH) receptor family. RPR4 was identified by PCR of rat pituitary cDNA, and a full-length clone was isolated from a rat olfactory bulb cDNA library. When RPR4 was functionally expressed in COS 7 cells, cyclic adenosine monophosphate (cAMP) production was stimulated by vasoactive intestinal peptide (VIP), pituitary adenylate cyclase activating peptides (PACAP-38 and PACAP-27) and helodermin, with equal potency. Peptide histidine isoleucine (PHI) and rat growth hormone releasing hormone (rGHRH) also stimulated cAMP production at lower potency. This suggests that RPR4 encodes a novel VIP receptor which we have designated the VIP₂ receptor. In situ hybridisation showed that mRNA for this receptor was present mainly in the thalamus, hippocampus and in the suprachiasmatic nucleus.

【 授权许可】

Unknown