Molecular cloning and expression of a cDNA encoding a receptor for pituitary adenylate cyclase activating polypeptide (PACAP)

FEBS Letters

Lutz, E.M.¹  Fink, G.¹  Morrow, J.A.¹  West, K.M.¹  Harmar, A.J.¹ 

[1] MRC Brain Metabolism Unit, University Department of Pharmacology and Royal Edinburgh Hospital, Edinburgh, UK

关键词: Pituitary adenylate cyclase activating polypeptide (PACAP);    G protein-linked receptor;    Vasoactive intestinal peptide;    Rat pituitary;    Rat olfactory bulb;    cDNA cloning;    ACTH;    adrenocorticotropic hormone;    cAMP;    adenosine 3':5'-cyclic monophosphate;    cDNA;    complementary DNA;    CGRP;    calcitonin gene related peptide;    CRF;    corticotropin releasing factor;    dNTP;    deoxynucleotide triphosphate;    DMEM;    Dulbecco's modified Eagle's medium;    GHRH;    growth hormone releasing hormone;    GLP;    glucagon-like peptide I;    mRNA;    messenger RNA;    PACAP;    pituitary adenylate cyclase activating polypeptide;    PCR;    polymerase chain reaction;    PHI;    peptide histidine isoleucine;    PTH;    parathyroid hormone;    RNase;    ribonuclease;    VIP;    vasoactive intestinal peptide;   

DOI  :  10.1016/0014-5793(93)80202-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned and sequenced a novel cDNA (RPR7) encoding a receptor for pituitary adenylate cyclase activating polypeptide (PACAP). RPR7 was identified by PCR of rat pituitary cDNA, and full-length clones were isolated from a rat olfactory bulb cDNA library. When expressed in COS cells, RPR7 was functionally coupled to increases in intracellular cyclic adenosine monophosphate (cAMP) in response to stimulation by PACAP-38, PACAP-27, vasoactive intestinal peptide (VIP) and peptide histidine isoleucine (PHI). The order of potency of these ligands was PACAP-38 ~ PACAP-27 > VIP > PHI, suggesting that the receptor corresponds to the pharmacologically characterised PACAP Type I receptor.

【 授权许可】

Unknown