Human apolipoprotein A‐II: complete nucleic acid sequence of preproapoA‐II

FEBS Letters

Brewer, H.Bryan¹  Lackner, Karl J.¹  Law, Simon W.¹ 

[1]Molecular Disease Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20205, USA

关键词: High density lipoprotein;    Apolipoprotein A-II;    Oligonucleotide probe;    Lipoprotein metabolism;    Nucleic acid;    sequence analysis;    apo;    apolipoprotein;    HDL;    high density lipoproteins;    SSC;    1 × SSC is 150 mM NaCl;    15 mM Na citrate;   

DOI  :  10.1016/0014-5793(84)80590-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete cDNA nucleic acid sequence of preproapolipoprotein (apo) A-II, a major protein constituent of high density lipoproteins, has been determined on clones from a human liver ds-cDNA library. Clones containing ds-cDNA for apoA-II were identified in the human liver ds-cDNA library using synthetic oligonucleotides as probes. Of 3200 clones screened, 4 reacted with the oligonucleotide probes. The DNA sequence coding for amino acids −17 to +17 of apoA-II were determined by Maxam-Gilbert sequence analysis of restriction fragments isolated from one of these clones, pMDB2049. The remainder of the cDNA sequence was established by sequence analysis of a primer extension product synthesized utilizing a restriction fragment near the 5'-end of clone pMDB2049 as primer with total liver mRNA. The apoA-II mRNA encodes for a 100 amino acid protein, preproapoA-II that has an 18 amino acid prepeptide and a 5 amino acid propeptide terminating with a basic dipeptide (Arg-Arg) at the cleavage site to mature apoA-II.

【 授权许可】

Unknown