Structures of fucose‐containing ceramide pentasaccharides from the plasma of blood group O Le(a−b−) nonsecretors

FEBS Letters

Dabrowski, Janusz¹  Kordowicz, Maria³  Egge, Heinz²  Pfannschmidt, Gerhard³  Hanfland, Peter³  Dabrowski, Ursula²  Peter-Katalinić, Jasna² 

[1] Max-Planck-Institut für Medizinische Forschung, Jahnstraße 29, 6900 Heidelberg, FRG;Institut für Physiologische Chemie der Universität Bonn, Nussallee 11, 5300 Bonn 1 Heidelberg, FRG;Institut für Experimentelle Hämatologie und Bluttransfusionswesen der Universität Bonn Heidelberg, FRG

关键词: Blood group;    Glycosphingolipid;    Human plasma;    Lewis negative (non) secretor;    Abbreviations and trivial names;    are essentially the same as in [3] : Gal(β1 → 4)[Fuc(α1 → 3)] GlcNAc(β1 → 3)Gal(β1 → 4)Glc;    lacto-N-fucopentaose III [19];    Fuc(α1 → 2)[Gal(β1 → 4)]GlcNAc(β1 → 3)Gal(β1 → 4)Glc;    lacto-N-fucopentaose IV;    amu;    atomic mass units;   

DOI  :  10.1016/0014-5793(84)81077-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A minor, Le^c blood-group inactive ceramide pentasaccharide double band fraction has been isolated from the plasma of blood group O Le(a⁻b⁻) nonsecretors. The two purified glycolipids were analysed by NMR spectroscopy, mass spectrometry and combined gas chromatography-mass spectrometry. The following structures could be established: GlcNAc(β1 → 3)Gal(β1 → 4)Glc(β1 → 1)Cer (I); Gal(β1 → 4) [Fuc(α1 → 3)]GlcNAc(β1 → 3)Gal(β1 → 4)Glc(β1 → 1)Cer (II). It must be concluded that at least part of the secretor gene-independent plasmatic H type 2 blood-group activity can be attributed to glyco-sphingolipid I, whereas substance II, originally detected in cancerous tissue, also occurs in the plasma of healthy individuals.

【 授权许可】

Unknown