Carbonic anhydrase located on cell surface increases the affinity for inorganic carbon in photosynthesis of Dunaliella tertiolecta

FEBS Letters

Miyachi, Shigetoh¹  Aizawa, Katsunori¹ 

[1] Institute of Applied Microbiology, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

关键词: Carbonic anhydrase;    Photosynthetic O2 evolution;    Subtilisin treatment;    Cell surface;    Dunaliella;    Marine alga;    CA;    carbonic anhydrase;    Diamox;    2-acetylamino-1;    3;    4-thiadiazole-5-sulfonamide;    high-CO2 cells;    algal cells grown in air enriched with 1.5–4% CO2;    low-CO2 cells;    algal cells grown in ordinary air (containing 0.04% CO2);   

DOI  :  10.1016/0014-5793(84)81013-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Low-CO₂ cells of Dunaliella tertiolecta had higher carbonic anhydrase (CA) activity on their surfaces than inside. The treatment with subtilisin completely abolished CA activity on the cell surface, while the activity inside the cells was not affected by this treatment. Correspondingly, the apparent K _m (NaHCO₃) for photosynthesis was increased two-fold, while V _max was not affected by this treatment. The apparent K _m(NaHCO₃) was lowered to the original level when CA was added to the suspension of subtilisin-treated cells. These results indicate that CA on the cell surface enhances the affinity for inorganic carbon in photosynthesis of D. tertiolecta.

【 授权许可】

Unknown