A novel type of crystallin in the frog eye lens

FEBS Letters

Luchin, Sergey V.²  Dolgilevich, Svetlana M.²  Gause, Georgyi G.²  Tomarev, Stanislav I.²  Krayev, Alexander S.¹  Skryabin, Konstantin G.¹  Zinovieva, Rina D.² 

[1]Institute of Molecular Biology, USSR Academy of Sciences, Moscow 117334, USSR

[2]Institute of Developmental Biology, USSR Academy of Sciences, Moscow 117334, USSR

关键词: Recombinant DNA;    DNA sequence;    Protein structure;    Ophthalmology;    Eye lens;   

DOI  :  10.1016/0014-5793(84)80508-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nucleotide sequence of a cloned DNA coding for the 35-kDa polypeptide of the eye lens of the frog Rana temporaria has been determined. The sequence without connectors and poly(A) tract is 889 nucleotides in length and shows no homology with sequences coding for other classes of crystallins; α-, β-, γ- or δ-crystallins. The sequence contains one reading frame 675 nucleotides in length, an apparently intact 3′-non-translated region with the polyadenylation signal sequence and a poly(A) tract; the 5′-non- translated region is lost along with part of the coding region; this accounts for about 1/4 of the total mRNA length. The secondary structure prediction according to the Ptitsin-Finkelstein method shows the presence of predominantly β-strands with only a few α-helical regions. We conclude that the 35-kDa polypeptide from the frog eye lens belongs to a new class of eye lens crystallins for which we propose the name ϵ-crystallin.

【 授权许可】

Unknown