Fluctuations in phosphoribosyl pyrophosphate levels in monolayer tumor cell lines

FEBS Letters

Pinedo, H.M.¹  Peters, G.J.¹  Laurensse, E.¹  Leyva, A.¹ 

[1] Laboratory of Biochemical Pharmacology, Department of Oncology, Free University Hospital, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands

关键词: Phosphoribosyl pyrophosphate;    Monolayer tumor cell;    Modulation;    Pyrimidine antimetabolite;    Purine;   

DOI  :  10.1016/0014-5793(84)81328-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The concentration of PRPP (phosphoribosyl pyrophosphate) measured in tumor cells grown in monolayer showed a large variation with the various harvesting methods examined, including trypsinization. This variation could be reduced by a 1-h incubation of trypsinized cells as a suspension in Dulbecco's medium. After this preincubation these cell suspensions were suitable for the study of modulation of PRPP. One μM methotrexate caused a 2–3-fold increase and 1 mM N-phosphonoacetyl-L-aspartate a slight increase, but inosine and deoxyinosine drastically reduced PRPP concentrations. 5-Fluorouracil had no effect. This study demonstrates that metabolic parameters such as PRPP concentrations can be studied conveniently in suspensions of cells which are commonly cultivated as monolayers.

【 授权许可】

Unknown