High tyrosine protein kinase activities in soluble and particulate fractions in bone marrow cells

FEBS Letters

Takeuchi, Fumito¹  Nakamura, Shun-ichi¹  Yamamura, Hirohei¹  Kondo, Hiroki² 

[1] Department of Biochemistry, Fukui Medical School, Matsuoka, Fukui 910-11, Japan;Department of Industrial Chemistry, Faculty of Engineering, Nagasaki University, Bunkyo, Nagasaki 825, Japan

关键词: Protein phosphorylation;    Tyrosine protein kinase;    Bone marrow;    Proliferation;    Differentiation;   

DOI  :  10.1016/0014-5793(84)81386-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

High tyrosine protein kinase activities were detected in soluble and particulate forms from bone marrow cells using synthetic peptide (Glu-Asp-Ala-Glu-Tyr-Ala-Ala-Arg-Arg-Arg-Gly) as a substrate. Total activity of tyrosine protein kinase was 2.4 times higher in the soluble fraction but the specific activity was slightly higher in the particulate one. Mg²⁺ or Mn²⁺ requirements of these two enzymes for maximal activity were quite different from each other. Physiological significance of these two forms of enzymes is briefly discussed.

【 授权许可】

Unknown