SDS—PAGE strongly overestimates the molecular masses of the neurofilament proteins

FEBS Letters

Weber, Klaus¹  Geisler, Norbert¹  Kaufmann, Eckhard¹ 

[1] Max Planck Institute for Biophysical Chemistry, D-3400 Göttingen, FRG

关键词: Neurofilament;    Molecular mass;    Gel electrophoresis;    Dodecyl sulfate;    Guanidine—HCl;    Intermediate filament;   

DOI  :  10.1016/0014-5793(84)81373-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Direct molecular mass determination of the three porcine neurofilament proteins (H, M and L) was performed in 6 M guanidine—HCl using analytical gel filtration and sedimentation equilibrium centrifugation. The results show that SDS—PAGE strongly overestimates the values of the ‘higher molecular mass’ components H and M. This discrepancy stems from the carboxyterminal extensions known to have unusual amino acid composition.

【 授权许可】

Unknown